Place it in the middle of a reaction sequence, between a SpongeBob Fail cue and a Vine Boom, so the fail sets the scene and the boom closes it. For a bigger payoff, end the sequence with an MLG Air Horn once the reaction has settled.
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This clip is a single human scream stretched across about three and a half seconds, holding one long vowel sound without words or other effects. It feels sudden, frantic and over the top, like a cartoon reaction to shock or panic, and it lands best when the exaggeration matches the moment.