Place it at the end of a failed attempt or a wrong answer, just before the reaction shot, then follow it with a Vine Boom to underline the fallout or an MLG air horn if you want the joke to escalate further.
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This is a short, sharp horn blast lasting about 1.6 seconds, with the brash, unmistakable tone of a car horn used for comic effect. It feels sudden, cheeky and attention-grabbing, like a playful toot meant to interrupt a moment. It comes from Berto on Nadie Sabe Nada.