Place it at the very end of an edit, after the closing line, so it closes the scene. Pair it with the Roblox Oof sound for a defeat first, then this clip, or put the Nope sound just before it when a character refuses to carry on.
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This is the Bop It XT toy announcing it is going to sleep in its bright, robotic game voice, a line lasting just under two seconds. The cheerful delivery against the tired message feels funny and deadpan, like the toy is giving up mid-game. It comes from the Hasbro Bop It XT electronic game, which plays the line when it powers down after a period of inactivity.