Put this clip at the reveal in a gag edit, after the Dun Dun Dun Sound Button and before the SpongeBob Fail Sound Button lands the payoff, then save the MLG Air Horn Sound Button for a wider group reaction scene later.
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This is a very short cartoon horn effect lasting about a tenth of a second, built from a medium squeeze of a clown bicycle bulb horn. It lands as a silly, startling little honk that turns an ordinary moment into a slapstick joke, perfect for punctuating a goofy entrance, a failed attempt or an exaggerated reaction without any dialogue or music underneath it.