Place this clip at the reveal itself, after the mistake is already clear to viewers. Follow it with the SpongeBob Fail sound for a deflated tag, or precede it with a Vine Boom to set up the bad news before this takes over.
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This six-second clip is the sung 'dame da ne' line from Baka Mitai, delivered slowly in a heavy, dramatic male vocal. It turns a listener toward mock sorrow, making small regrets feel painfully earnest and funny at the same time. It comes from the karaoke song performed by Kazuma Kiryu in Yakuza 0 from the Yakuza game series.