Place it immediately after the mistake in your timeline, then follow it with a wrong answer buzzer for a second fail or a Minecraft oof if a character just took damage.
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This 2.3-second voice clip is the word I'm sorry spoken by a Dark Souls carving item in a hollow, echoing voice. It feels deadpan and uncanny, sincere one moment and sarcastic the next, which makes the apology land as both remorseful and funny. It comes from Dark Souls, where carvings play preset messages.