Place it at the end of a reveal, after the payoff is shown, so it closes the scene rather than opening it. Lead in with It Was At This Moment He Knew to set up the turn, then follow this clip with Darth Vader Nooo for a defeated reaction.
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A six-second voice clip of Thanos delivering his line in a slow, calm, deeply satisfied tone, with his gravelly voice carrying the whole moment. It feels darkly amused and quietly triumphant, like a villain enjoying a win he expected all along. The line comes from Thanos in the Marvel films.