Place it at the end of a reveal, after the bad news has sunk in, so it acts as the final reaction. Follow it with Emotional Damage for a bigger comic fallout, or lead into it with Sad Meow Song when the scene needs a slower build-up first.
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Duane is a brief 2.5-second meme clip centred on a bruised, aching heart, the feeling its uploader summed up in Portuguese as a heart left hurt. It lands as sad and a little melodramatic, the kind of small, wounded reaction that makes a disappointment feel bigger than it is, without turning it into a full breakdown.