Place this clip immediately after the on-screen accusation in your edit, then follow it with the Bruh Soundboard Button for the stunned reaction from everyone else. Keep the sequence accusation, denial, reaction, and do not add another voice line between those three beats.
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A short Portuguese voice clip, about three seconds long, delivers the defensive line 'Eu nao, eu sou virgem' — roughly 'Not me, I'm a virgin.' The delivery feels awkward, earnest and unintentionally funny, turning a blunt personal denial into a quick reaction that lands like an embarrassed confession rather than a serious argument.