Place it right after the visual reveal of a fail, before any commentary starts. For a bigger letdown build-up, lead in with the Womp Womp Fail Sound Button, then use this clip as the final tag, or follow it with the SpongeBob Boowomp Sound Button for a second deflated beat.
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This is a very short clip, just over one second long, of a high-pitched anime-style girl's voice breaking into an exaggerated cry. The sob is brief and theatrical rather than genuinely distressed. It feels funny and pitiful at once, turning a small disappointment into an over-the-top sad reaction that listeners instantly read as playful, mock-upset whining instead of real tears.