Place it at the very end of a fail sequence, after the mistake is visible and before any reaction clip. Follow a Roblox Oof with it for a cartoon death, or lead into a FNAF Jumpscare Scream for a horror-game fail.
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This short game-over cue plays a brief game-style sting that signals a failed run in just under four seconds. It feels sudden and anticlimactic, turning a loss into a small comic beat instead of a serious setback. The clear finish makes it easy to recognise even without any picture on screen.