Place it right after the impact moment in your edit, then let the reaction shot play out before the next gag. Pair it with a punch sound first for the hit, then a SpongeBob fail sting to turn the pain into a joke.
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This is a short, raw human scream lasting under a second, with a strained, high-pitched cry that sounds like someone reacting to sudden pain. It feels startling and over-the-top, more comic than frightening, so it lands as a sharp jolt rather than a slow build of fear.