Place it near the end of a build-up, just after Metal Gear Alert and before Cartoon Chase Music, so the warning hands the scene to the pursuit. Let the alert set the danger, use this clip as the turning point, then let the chase music carry the escape forward.
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This short spoken clip is a frantic, breathless warning shouted as a dangerous person slips away and needs to be stopped. The voice sounds urgent and alarmed, turning a simple escape into an immediate emergency. It creates a tense, panicky feeling that makes a listener expect a chase, a confrontation, or someone to act before it is too late.