Put this clip at the very end of a joke beat in your edit, after the spoken line finishes. For a bigger flop, follow it with the Vine Boom Sound Effect, or swap the order and lead with the SpongeBob Fail Sound Effect before returning to dialogue.
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This clip is a 0.36-second rimshot, a quick snare and cymbal ba dum tss sting played as a drum accent. It feels instantly funny and deliberately cheesy, turning even a weak pun into an obvious joke. It comes from the long-established comedy cue used in live performance and television after a punchline.