This clip comes from League of Legends, Riot Games' long-running multiplayer online battle arena game. The defeat sound plays when a match ends in a loss, making it instantly recognizable to players. Because the game has had a huge online audience for years, the audio spread naturally into streams, YouTube edits, and short gaming memes. It remains popular because the cue is brief, clear, and universally readable: the moment it plays, viewers understand that the play, round, or entire match went badly.