Place it right after the request or suggestion it rejects, so the refusal closes that moment in the edit. For a longer back-and-forth, follow it with No No Wait Wait to show the other person pleading, then end on Wrong Answer Game Show when their next idea fails too.
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This is a very short clip of a man saying a single firm 'No' in a clear, flat voice, lasting less than half a second. It lands as blunt, funny and final, with no hesitation or softening to take the edge off the refusal. That abruptness makes it feel like a door closing on a request, perfect for a quick, unmistakable rejection.