Place it in the middle of a fail sequence, after the setup and just before the impact, then follow it with a Roblox Oof for the landing and a No God Please No for the reaction shot that follows.
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This is the short falling sound from Super Mario 64, lasting about two seconds as Mario drops with a sliding, descending cry. It feels suddenly weightless and comic, like a cartoon plunge that keeps going down. Taken from the Nintendo 64 game, it carries the bright, playful panic of a missed jump or a long fall off a ledge.