Put it at the peak of the scene, after a slow build and just before the reveal lands. Start with Metal Gear Solid Alert to raise the tension, then let this clip hit, and follow it with Evil Laugh while the reaction is still fresh.
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This is a short, roughly two-second jumpscare scream built for a sudden shock, with a sharp, high-pitched shriek that hits fast and cuts off just as quickly. It feels startling and unsettling, the kind of burst that makes a listener flinch before they have time to react. Use it sparingly, because its whole impact comes from arriving without warning.