Put it in the middle of a meme edit, after a light setup and before the final reveal. Pair a Goofy Ahh Sounds clip first for the normal opening, then follow this cue with a Scream Meme Sound at the darkest image to complete the escalation.
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This is the short Phase 2 cue from the Mr. Incredible Becoming Uncanny meme, a tense musical sting lasting about three seconds with no dialogue. It feels ominous and uneasy, like a normal moment is sliding into something disturbing. It comes from the viral format using stills of Mr. Incredible from The Incredibles, where each numbered phase grows more unsettling.