Place it in the middle of an escalating edit, after a lighter setup and before the final reveal. Start with the Emotional Damage Meme, follow it with this clip, then close with the Scream Meme Sound for the peak moment.
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This short clip is the phase 3 audio from the Mr Incredible Becoming Uncanny meme, a brief, dark and distorted sound that lasts under four seconds. It feels eerie and uncomfortable, like a light joke sliding into something wrong. It comes from the escalating meme built around images of Mr Incredible from The Incredibles, where each phase grows more disturbing.