Place it right after the on-screen mistake, then follow it with the awkward moment sound for the reaction beat. If the character needs an entrance first, lead in with the teleport sound and let this clip take the final spot in the sequence.
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This roughly ten-second clip is built around Mr. Popo speaking in a calm, low voice, with his delivery carrying the whole moment. It feels unsettling and darkly funny, because that steady tone sits oddly beside the serious stare people associate with the character during a tense exchange.