Place it at the end of a failed attempt, after the build-up has peaked, and let it close the scene. For a bigger payoff, lead in with Sudden Suspense, then follow this clip with Nelson's Ha-Ha! for a second laugh, or Wrong Answer Game Show when the failure was a bad guess.
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This short 4.4-second clip opens with a drawn-out villainous laugh, 'Mwhahaha,' followed by the flat, dismissive line 'That was pathetic.' The exaggerated laugh and cold put-down give it a smug, theatrical feel, like a cartoon villain enjoying someone else's failure. It lands as funny and cutting at once, perfect for teasing a weak effort without saying much yourself.