Place it right after the clip that creates the false pairing, then follow it with Oh My God Wow for the group's reaction, or open with No No Wait Wait when you need a stronger stop before it. Keep it as the centre of the exchange, not as background filler.
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This is a short Portuguese voice clip, just over a second long, in which a speaker plainly states that they are not a couple. The delivery is direct and slightly exasperated, turning a simple denial into an awkward, funny correction that lands best when someone has just made the wrong assumption about a relationship.