Cut to this clip on the reaction shot, not the injury itself, then follow it with a sadder payoff sound. Pair it after a short impact effect and before the SpongeBob Sad Song Sound Button for a failed plan that keeps getting worse.
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This clip is a stretched-out male voice groaning "oooh" in pain, held for just over five seconds. It sounds like an overacted telenovela reaction to hurt or bad news, which gives it a funny, melodramatic and slightly awkward feeling instead of real distress.