Put it at the end of an introduction sequence, just after the person on screen has been shown, then follow it with the awkward moment sound to underline the reaction. For a sillier cut, place the anime ahh sound before it as a teasing build-up.
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This is a very short voice clip, lasting about 1.4 seconds, in which Phil says that he is a fem boy in a light, matter-of-fact tone. It feels playful and cheeky rather than dramatic, like a quick self-introduction meant to catch someone off guard. The delivery makes it easy to recognise and hard to take seriously, which is exactly why it works as a joke.