Place it as a beat between Popee scenes, then follow with Anime Ahh for a fan reaction and Awkward Moment when the joke stalls, keeping the sequence centred on the characters.
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This is a very short sound effect from Popee the Performer, lasting just over half a second and built around a single clipped burst. It feels odd, playful and slightly unsettling, like a tiny jolt from the show's strange circus world. That quick, offbeat character makes it easy to recognise for fans of the series.