Place it near the end of a short fail or farewell edit, after the moment of disappointment has already happened, then follow it with Sad Meow Song for a softer second beat or Emotional Damage for a sharper comic turn.
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This is a short, slow melody from Super Mario RPG, lasting about nineteen seconds, with a gentle, downcast tune that hangs on each note. It feels wistful and quietly heartbreaking, like a small defeat after a long effort, and it works best when the mood needs softening rather than a joke. The music comes from Super Mario RPG.