Place it right on the reveal or failed attempt, then follow it with Anime Ahh for a comic reaction, or cut to Awkward Moment Sound Effect when the joke falls flat. Keep it as the peak of the scene, with smaller reactions before it and a punchline sound after.
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A single anime-style girl lets out a brief, shrill scream that climbs fast and stops suddenly, lasting under two seconds. It carries pure startled panic, loud and theatrical enough to make a small shock feel huge. That cartoonish alarm gives fails and reveals a playful, overblown edge.