Place it right at the reveal in a prank edit, after the chocolate is tasted and before the reaction shot. Pair a Goofy Yell just before it for a buildup, then follow with Oh No No No Laugh once the joke lands and everyone recovers.
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This clip is a single human scream, just over half a second long, holding one stretched 'ah' at full volume. It feels sudden, silly and over the top, like panic played for laughs rather than real fear. The raw, unpolished delivery makes the outburst land as a joke, a shock or a mock horror reaction in a single breath.