Place it as the payoff after a calm setup, then follow it with a chicken on tree screaming clip for a second reaction and a scream meme sound to close the gag before the next scene starts.
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This clip is a single sheep scream lasting about four and a half seconds, with the animal's bleat rising into a startled, human-like cry. It feels absurd and startling at once, funny because the sound is so out of place coming from a sheep, and it works as a sudden jolt in a quiet scene.