Put it at the peak of a tense scene, after a slow Dun Dun Dun build and just before the reveal, then follow it with a Vine Boom to underline the reaction and reset the pace for the next joke.
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This is a brief, high-pitched screamer sound lasting under a second, built around a sudden shriek that hits fast and cuts off just as quickly. It feels startling and jarring, the kind of sharp shock that makes people flinch before they have time to laugh.