The laugh comes from Sam Raimi's Spider-Man 2 from 2004, in a scene where Peter Parker asks J Jonah Jameson, played by J K Simmons, for a pay advance. Jameson first treats it as serious, then breaks into prolonged laughter. The scene was clipped and shared for years as a reaction image and video on forums and social media, often captioned with requests no one would grant. Its lasting appeal is Simmons's committed performance, which turns a simple no into a complete comedic dismissal.
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J Jonah Jameson's long hysterical laugh from Spider-Man is used as a reaction sound when someone says something ridiculous, delusional or not serious at all.
The clip is J Jonah Jameson, Peter Parker's newspaper boss, bursting into a long, escalating fit of laughter after thinking he has heard a serious request. It starts with a short chuckle, pauses as if he might answer, then erupts into loud, wheezing, almost tearful laughing that keeps going far longer than expected. The humour comes from that deadpan setup and total overreaction, making the other person feel completely dismissed. Viewers instantly read it as mockery, disbelief and a refusal to take the moment seriously at all.