Put it last in the edit, after the final reveal, with a Bruh sound just before it to set up the defeat and an Emotional Damage sound after it to close the sequence.
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This is a short three-second spoken meme line that states the end has come. It feels abrupt and final, like a curtain dropping on a joke that has run out of road, and it works best as a deadpan punchline for a moment that is already over.