Place it at the exact frame of a reveal or mistake, after the setup line and before the reaction shot. Pair it before a vine boom sound for a bigger follow-through, or follow it with a ding sound effect for a lighter close.
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TKB is a short sound effect clip lasting just over two seconds, built around a single brief audio hit with no speech or music. It gives a listener a quick, punchy jolt, useful for marking a sudden beat rather than building a longer mood or scene.