Place it at the very end of a failed attempt, after the last mistake is shown, so it closes the sequence. Follow it with the Lego Yoda death sound for a second loss, or lead into it with the Mortal Kombat fatality sound when a game defeat needs a bigger finish.
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A Portuguese spoken line lasting about eleven seconds, stating plainly that everyone has died and it is all over. The blunt wording gives it a darkly comic, resigned feeling, as if the speaker has already accepted a hopeless ending. It works as a deadpan verdict on a failed plan, a lost game or a conversation that has gone completely wrong.