Place it at the end of a slow build-up, just before the payoff lands, then follow it with Vine Boom for a sharp punch. For a cartoon-style reaction, put Anime Wow first and let this clip carry the moment that follows.
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This short sound effect is built around one elongated vocal-style 'toonnnooooo', lasting about 2.8 seconds in total. The stretched, drawn-out delivery gives it a playful, overdone feeling, as if a simple reaction is being held a little too long for comic effect. It works as a light, silly accent rather than a serious cue.