Place it at the exact cut where a bad idea gets revealed in your edit. Start with the No No Wait Wait button to build the objection, follow immediately with this clip as the payoff, then add a cartoon slip for a physical gag after.
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A very short voice-changed clip where a distorted voice blurts 'uh no' followed by rapid 'poi poi poi' sounds. At just over half a second it lands as a frantic, silly refusal, giving a funny and slightly panicked feeling that works as an instant no-way reaction.