Place it at the end of a reveal, after the twist is clear, so it closes the scene instead of competing with dialogue. Build tension first with Sudden Suspense, then let this clip take the punchline, and follow it with Nelson's Ha-Ha! for a second laugh that keeps the joke rolling.
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This is an eight-second demonic laugh, a loud, mocking cackle credited as the laugh of D. Montes in its second version. It feels wicked, teasing and over the top, like someone revelling in another person's bad luck. The exaggerated, villainous tone makes it land as a cruel joke rather than a friendly giggle.