Place it as the punchline after the offending line, then follow it with Eww Brother Eww for a second reaction, or with Nelson's Ha-Ha for a laugh that keeps the joke going.
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This is a very short spoken clip, lasting just over a second, in which Gil Brother delivers the Portuguese insult 'Você está defecando pela boca' in a blunt, confrontational voice. It feels crude, mocking and deliberately over the top, like a verbal slap meant to shut down nonsense. The line comes from Gil Brother, the Brazilian comedian behind the delivery.