Put it at the very start of a wake-up gag, then let an air horn follow it to keep the gag going, and finish with a drum sting once the sleeper reacts. That order builds the joke in three beats without crowding the edit.
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This is the brief opening vocal from System of a Down's Chop Suey!, lasting under a second and built around a single shouted wake-up line over a sudden musical hit. It feels jarring, loud and instantly dramatic, like an alarm going off in the middle of a quiet scene. The line comes from the band's 2001 song on the album Toxicity.