Place it just before a reveal or a punchline cut, then follow it with a Vine Boom for the payoff and an Anime Wow when the reaction lands. Keep it near the start of an edit so the next gag has room to breathe.
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This is a very short whistle sound, lasting well under a second, with a clear, light tone that cuts through a quiet moment. It feels playful and a little cheeky, like a quick nudge to get someone's attention without saying a word.