Put it at the very end of a sequence, after the mistake has already happened. Lead with the Classic Error Sound to set up the failure, then let this clip close the scene. Follow it with nothing else, so the ending stays clean and the viewer knows the session is over.
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This is a short Windows-style shutdown clip lasting about five seconds, built around the familiar power-down chime that signals a computer turning off. It feels abrupt and final, with a dry, slightly comic edge that makes an ending land harder than a plain fade-out.