Place it right after the wrong answer is revealed, then follow it with a Dun Dun Dun cue to underline the loss before moving on. For another fail in the same round, pair it with a SpongeBob Fail clip instead, keeping the penalty as the first sound in the sequence.
Über diesen Sound
This is a short sound effect lasting about 4.6 seconds, built around the idea of a point being taken away. It works as a quick penalty cue for a wrong answer, a failed attempt or a score going down. The feeling is a small, deflating sting of disappointment, suited to a missed chance without turning the moment into a big defeat.