Place it just after the reveal in a short edit, with Sudden Suspense building the moment before it and Nelson's Ha-Ha! following it to turn the scene into laughter. That order turns a small confrontation into a complete joke, with it as the middle beat.
Über diesen Sound
This is a short 2.5-second growl by JDG, a low, gruff 'grou grou' delivered in a deliberately mean, villainous voice. It sounds like a playful warning from someone pretending to be furious, which makes it funny rather than frightening, and it works as a quick, exaggerated threat for a silly confrontation.