Place it early in a scene before the big reveal, then follow it with Dun Dun Dun, and save Vine Boom for the moment the scare or punchline finally lands.
Über diesen Sound
This is a roughly six-and-a-half-second clip of a person breathing close to the microphone, with each inhale and exhale clearly audible. It feels intimate and slightly uneasy, like catching someone mid-breath in an empty room, and that closeness is what makes it work as a reaction.