Place it immediately after the failed moment in your edit, then follow it with a wrong answer sting to close the joke, or open with a cartoon slip before the mishap and let it answer that setup.
Über diesen Sound
This is a short, roughly 1.6-second vocal groan, a single drawn-out 'agh' delivered in a frustrated, exasperated tone. It lands as an instant sigh of annoyance, the kind of sound that makes a small failure feel bigger and funnier, without needing any extra words or explanation.