Place it right at the reveal in a fail edit, then follow with a bruh soundboard button for the flat reaction, or an emotional damage sound button when the loss needs a bigger second beat to close the scene.
Über diesen Sound
This is a very short voice clip of someone saying “Ah não... Aí não!” in Portuguese, the whole line delivered in under a second. It lands as a mix of dismay and protest, like watching something cross a line you cannot undo, which makes the reaction feel funny, awkward and instantly sympathetic rather than angry.