Place it right after the reveal or the mistake, then follow it with a Vine Boom for impact and finish with a SpongeBob Fail for the aftermath, keeping it as the middle beat of the sequence.
Über diesen Sound
This clip is a drawn-out human scream on a single 'ahhh', with the voice held through the whole sound and no words in it. It feels panicked and over the top, like someone reacting to a sudden shock, which makes it funny rather than frightening when it lands in a joke or a reaction video.