Put it at the end of a short fail sequence, after It Was At This Moment He Knew sets up the mistake, then follow it with clapping to sell the audience reaction.
Über diesen Sound
This is a short, low, resonant brong lasting about a second and a half, the kind of heavy cartoon thud that lands after a clumsy move. It feels funny and a little overblown, turning a small mishap into a big, deliberate payoff. It comes from the Goofy cartoon How to Dance, released in 1953.