Place it just before the reveal in a short edit, then follow the noticing beat with a bell ding to mark the detail, and save an anime wow for the payoff when the joke fully lands.
Über diesen Sound
This 0.8-second clip is a bright, chirpy game cue that pops up when a character spots something unexpected. Its light, playful lift makes a small discovery feel funny and endearing rather than dramatic. It comes from Animal Crossing, where short emote cues show mood without words.